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KMID : 0848120150400040223
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International Journal of Oral Biology
2015 Volume.40 No. 4 p.223 ~ p.228
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Apoptotic Effects of 6-Gingerol in Human Breast Cancer Cells
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Kim Hyun-Woo
Oh Deuk-Hee
Koh Jeong-Tae
Lim Young-Chai
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Abstract
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6-Gingerol exerts anti-tumor effects in various cancer cell models. We evaluated the effect of 6-gingerol on the growth of MCF-7 breast cancer cells and MCF-10A breast epithelial cells to determine whether any growth-inhibitory effects found were attributable to apoptosis, and to elucidate the underlying mechanism of action. 6-Gingerol inhibited the viability of both cell lines in a dose- and time-dependent manner; however, the degree of inhibition was greater in MCF-7 than MCF-10A cells. By flow cytometry, induction of dose- and time-dependent apoptosis was found, and the magnitude of apoptosis was also markedly greater in MCF-7 than MCF-10A cells. Expression of caspase-3 and poly (ADP-ribose) polymerase (PARP) was observed in MCF-7 cells treated with 6-gingerol, and further cleavage of PARP occurred in these cells. We suggest that 6-gingerol induces apoptosis in human breast cancer cells mainly by promoting caspase-3 expression and subsequent degradation of PARP.
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KEYWORD
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6-Gingerol , Apoptosis , Caspase-3 , PARP , Human Breast Cancer Cells
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